Protein_Domain

Part:BBa_K1692028:Design

Designed by: Charles Calvet   Group: iGEM15_Stanford-Brown   (2015-09-14)


cotZ-aeBlue-CIPA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 54
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1212


Design Notes

This part was ligated into the pSBbs1c bacillus backbone from the LMU Munich 2012 iGEM team's Bacillus Bio Brick Box (https://parts.igem.org/wiki/index.php?title=Part:BBa_K823023)


Source

cotZ from the LMU munich 2012 iGEM team (https://parts.igem.org/Part:BBa_K823032). aeBlue from iGEM12 Uppsala (https://parts.igem.org/Part:BBa_K864401), CIPA from our lab

References